BioArray^® Single Round RNA Amplification and Labeling System (ENZ-42420-10)

• Convenient all-in-one system containing Biotin-labeled nucleotides
• Widely used and compatible with several microarray platforms including the Affymetrix GeneChip^®
• Highly sensitive analysis of eukaryotic mRNA with minimal starting materials
• Increased % Present calls on gene expression microarrays
• Improved data quality metrics and higher aRNA yields from a wide range of input RNA
• Highly reproducible results (r² = 0.997)

Detection limit: 2 pg/ml
Detection range: 0 to 250 pg/ml

Free Osteoprotegerin (human) Detection Set [For ELISA Application]
Prod. No. APO-54N-028

For the quantitative determination of human free osteoprotegerin (not bound to RANKL or TRAIL) in cell culture supernatant, plasma and serum.

Figure: Netrin-1 (human) (recombinant) (Prod. No. ALX-522-100) mediates commissural axon outgrowth of dorsal spinal cord cells from rat embryos.

Also available:

Netrin-1 (mouse) (recombinant)   Prod. No. ALX-522-124
Netrin-1 (chicken) (recombinant) Prod. No. ALX-522-106

• First commercial source for IL-33 from rat!
• All IL-33 proteins from human and mouse are positively tested to activate the ST2-dependent NF-κB pathway!
• ≥90 % pure (SDS-PAGE)
• Endotoxin-free

IL-33 (human) (recombinant)             Prod. No. ALX-522-098
IL-33 (human) (recombinant)             Prod. No. ALX-201-363
IL-33 (human) (recombinant) (His)   Prod. No. ALX-201-350
IL-33 (mouse) (recombinant)             Prod. No. ALX-522-101
IL-33 (mouse) (recombinant) (His)   Prod. No. ALX-201-361
IL-33 (rat) (recombinant)                    Prod. No. ALX-522-125

Figure: C) Rat prostate carcinoma cells expressing GFP-β-tubulin (green) were stained with Hoechst (DNA, blue) and centrosomes were visualized using the Monoclonal Antibody to γ-Tubulin (TU-30) (DY547), Prod. No. EXB-1C-465 (red).
D–F) Mitotic spindle in 3T3 mouse fibroblasts (D – metaphase, E – anaphase, F – telophase) as detected by the Monoclonal Antibody to γ-Tubulin (TU-30) (DY647), Prod. No. EXB-1D-465 (red), anti-α-tubulin (green) and DAPI (DNA, blue).

Also available unconjugated:

Monoclonal Antibody to γ-Tubulin (TU-30)  Prod. No. EXB-11-465

Detection limit: 30 pg/ml
Detection range: 0 to 32 ng/ml

Visfatin (human) ELISA Kit  Prod. No. AG-V0523

Excellent Quality - High Sensitivity - Batch-to-Batch Reproducibility!

For the quantitative determination of visfatin in human plasma, serum or cell culture supernatants.

PI-103  Prod. No. ALX-270-460

Purity ≥ 97 % (HPLC)

Nesfatin-1 is an important satiety molecule associated with melanocortin signaling in the hypothalamus.

MAb to Nesfatin-1 (Nesfaty-1)  Prod. No. ALX-804-854

• Recognizes mouse and rat nesfatin-1 and its precursor nucleobindin-2
• Immunogen: Recombinant rat nesfatin-1 (aa 25-106) (Prod. No. ALX-522-118)
• Clone: Nesfaty-1
• Application: Western blot
• Purity: ≥95% (SDS-PAGE), Protein G-affinity purified

Figure 1: Beclin1 and Ambra-1 (activating molecule in Beclin 1-regulated autophagy) co-localize in mammalian cells.

Ambra-1 is a positive regulator of Beclin1-dependent autophagy and has a crucial role in embryonic nervous system development.

PAb to Ambra-1 Prod. No. CVL-PAB0224

• Recognizes human, mouse and rat Ambra-1
• Immunogen: Synthetic peptide corresponding to the N-terminus of mouse Ambra-1
• Applications: IHC, IP, Western blot

CyTRAK Orange™  Prod. No. BOS-889-004

Key Benefits:

Binding/Labeling
one step visualization of nuclear and cytoplasmic boundaries through labeling with differential intensities -> one fluorophore doing the work of two
for LIVE and FIXED cells
non-crosslinking

Spectral 
no excitation by UV/red lasers
no emission overlap with co-excited "green" fluorophores
can be used on a wide range of imaging platforms
single scan with GFP/FITC/Cy2, then dual scan with red excited dyes for more parameters
no need to wash out

Chemical
rapid staining
low photobleaching
membrane-permeable -> no lyse or wash protocols needed
assay buffer compatible

Total Antioxidant Capacity Assay Kit Prod. No. ABL-TAC-Peroxyl-KI01

• Inhibition assay of chemiluminescence caused by peroxyl radicals.
• Includes peroxyl radical generator that mimics natural lipid peroxidation.
• Applicable to serum, semen plasma, CSF, tissue homogenates, urine, tea, fruits, wines, juices, botanical extracts.

Natural substances play an important role in the development of new, innovative ingredients and new chemical entities (NCEs). The number of naturally ocurring substances is quite high and may be increased multi-fold by combining parts of natural substances with synthetic fragments. However, companies developing NCEs often face the problem, that multi-gram to kilogram quantities of fermentation products, required for their pre-clinical trials, are difficult to obtain.

Dolder & Novartis BioPilotplants close this gap, offering these products in bulk quantities. All research quantities are sold through ALEXIS^® Corporation.